News, Events and Workshops

The list of news and events is by no means a comprehensive one, so if you have anything you'd like to add email Sharon at sharon.lequeux@anatomy.otago.ac.nz

  • News from 2016 and earlier


  • News from 2017:


    No March Monthly Microscopy Meeting

    There will be no microscopy meeting this March as the last Thursday of the month is too close to the Easter holidays. The next meeting will be on Thursday the 3rd of May at 9:00AM in the seminar room of the Benham (Zoology) building, where we will hear from Dr. Matteo Demurtas (Department of Geology) about TKD techniques in the SEM.

    "Application of Transmission Kikuchi Diffraction to nanogranular geological materials"

    Nanoparticles (<1 micron) are found widely in tectonic fault zones and have successfully been reproduced in laboratory experiments. Although various deformation processes have been proposed to form nanoparticles in faults, their origin and influence on fault mechanical behavior is still poorly understood. Understanding the deformation processes that produce nanoparticles in natural and experimental faults requires an understanding of their grain sizes, shapes, crystallographic orientations and grain boundary structures. However, conventional microanalytical techniques often lack the spatial resolution to understand the crystallographic characteristics of nanograins. Transmission Kikuchi Diffraction (TKD) in the SEM was developed several years ago (Trimby, 2012, Ultramicroscopy) and has been successfully used to study the crystalline structure of nanostructured metals and alloys. TKD allows crystallographic orientation mapping to be performed at high spatial resolution (down to few nm) over relatively large sample areas (103 m2). In this talk I will introduce the technique and present results of TKD analysis performed on the Zeiss Sigma VP Field-Emission-Gun Scanning Electron Microscope in the OCEM in Otago. These are among the first ever results on geological samples collected from fault zones. The TKD analysis allows us to better constrain the deformation processes that occur in fault zones during earthquakes, including the activity of dislocation creep and grain boundary sliding aided by diffusion creep.


    Imaging Unit Manager Vacancy

    University of Otago is looking for an Imaging Unit Manager (who will manage the EM, confocal, histology and flow cytometry units). You can find out more about the position on Otago's 'Current Vacancies' page.


    Paper of the Month


    Strauss, M., Jayawardena, N., Sun, E., Easingwood, R.A., Burga, L.N. and Bostina, M. (2018) Cryo-Electron Microscopy Structure of Seneca Valley Virus Procapsid. Journal of Virology, doi.org/10.1128/JVI.01927-17.



    Dr. Alasdair McDowall Visits the OCEM

    The OCEM was lucky enough to be paid a visit by Dr. Alasdair McDowall and his wife Leta McDowall during which he told us about the Nobel ceremony and let us play with his medal. Alasdair played a key role in optimising vitrification (which made cryo-EM possible) and was the first-author several of the early papers from the Dubochet lab that the Nobel Prize recognized. McDowall was so integral to this work that Dubochet invited McDowall and Leta to attend the 2017 Nobel ceremonies and gave one of the three replica medals offered to each Nobelist to McDowall.


    alasdair pizza sharon medal
    alasdair medal sharon medal
    medal1 medal2



































    IMC19

    The 19th International Microscopy Congress (IMC19) will be held 9-14 September 2018 in Sydney. Held every four years, and for the first time in Australian in many decades, this is a special AMMS (Australian Microscopy & Microanalysis Society), held under the auspices of IFSM. The congress will attract over 3,000 scientists working within and outside the microscopy sector. There will be a large interactive trade exhibition where leading suppliers are set to launch ground-breaking new instruments.


    Monthly Microscopy Meeting

    The next meeting is at 9:00AM Thursday the 1st March in the seminar room of the Benham (Zoology) building.

    Antimicrobial Coatings on Hospital Touch Surfaces: How would you tell if it works?
    Dr. Aleksandra Gardecka, University of Canterbury

    antimicrobial coating Nosocomial infections are a serious problem. There is a huge amount of interest in antimicrobial materials that could be used for coatings in health care facilities. The concept is that the door handles, bed rails, switch plates and other touch surface could be coated with antimicrobial materials and thus reduce the transmission probability. In the Advanced Energy and Material Systems lab in Canterbury we develop robust coatings for stainless steel touch surfaces exhibiting excellent antimicrobial properties both in UV and visible light, substantially reducing amount of present bacteria therefore contributing in prevention of nosocomial infections.


    AsCA 2018/CRYSTAL 32 2-5th December 2018

    AsCA 2018/CRYSTAL 32 is a combined conference of the Asian Crystallographic Association (AsCA) and the Society of Crystallographers in Australia and New Zealand (SCANZ) being held in Auckland on the 2nd-5th December 2018. Dr. Mihnea Bostina (Academic Director of OCEM) will be chairing a symposium 'Applications of cryo-EM to structural biology'.


    Basic Microscopy Skills Workshop

    This week the Leica Product Manager, Hazel Bengan, is in Dunedin. She is available on Tuesday & Wednesday to discuss any Microscopy requirements you may have. This would be by appointment and most suitable as a refresher for your Technicians and Post Grad Students who may have some current practical requirements. Bio-Strategy are offering this opportunity in recognition of the support of Leica Microscopy Products at this University and want to help those using Microscopes to get the best from their valuable instruments.

    WHEN: Afternoon Wednesday 31st January 2.00-4.00pm

    WHERE: Bio-Strategy Lab, Suite 24, Ground Floor, Centre for Innovation Bldg. Uni Otago

    WHAT: A Leica Teaching DM750 Microscope and ICC50 W Camera will be available to train on for example,
    - Widefield Microscopy
    - upright and inverted microscope configurations
    - Contrast Methods including Koehler Illumination
    - Factors in selecting the right microscope
    - Microscope Cleaning and Maintenance
    - Digital Imaging (microscope cameras and software such as Leica LAS Core, LAS EZ, LAS AirLab)

    Email pippa.newstead@bio-strategy.com with time that suits, who will come from your Group and any specifics you want to learn and we will get back to you. Appointments will be on a first reply basis and approx. 1 hour each for a small group.


    Monthly Microscopy Meeting

    The next meeting is at 9:00AM Thursday the 26th of October in the seminar room of the Benham (Zoology) building. The OCEM intends to purchase an SEM to bring new capabilities to the unit. In order to decide on the optimal configuration, we have organised several presentations by providers. This month we will hear from Ian Cotton from Hitachi (Canada), and from Graeme Jones from NewSpec (Australia).

    There will also be a sales presentation at 1.00PM on Thursday the 26th October in the Hunter Centre (seminar room TBC).



    Monthly Microscopy Meeting

    The next meeting is at 9:00AM Thursday the 31st of August, in the seminar room of the Benham (Zoology) building. The OCEM intends to purchase an SEM to bring new capabilities to the unit. In order to decide on the optimal configuration, we have organised several presentations by providers. This month we will hear from Kamran Khajehpour from AXT, an Australian based supplier who, amongst at very wide range of EM preparation equipment, offer the Tescan range of scanning elecron microscopes and the Delmic range of CLEM platforms and cathodoluminescene detectors.

    There will also be a sales presentation at 1.00PM on Thursday the 31st August in room 120 of the Hunter Centre.

    "Next Generation Microscopy Solutions for Life and Material Science Applications"


    Paper of the Month

    Miles, L.A., Burga, L.N., Gardner, E.E., Bostina, M., Poirier, J.T. and Rudin, C.M. (2017) Anthrax toxin receptor 1 is the cellular receptor for Seneca Valley virus. The Journal of Clinical Investigation, doi.org/10.1172/JCI93472.

    Seneca Valley Virus

    Cryo-EM Workshop - 24th July 2017

    Short Introduction to cryo-EM
    1030AM on Monday the 24th July and Tuesday the 1st of August 2017
    Room 207, Microbiology building. Enquires to mihnea.bostina@otago.ac.nz.



    Monthly Microscopy Meeting

    The next Microscopy Monthly Meeting is at 9:00AM Thursday the 27th of July, in the seminar room of the Benham (Zoology) building.

    Steve Seo
    Department of Anatomy
    "Understanding a synaptic connector in the healthy and diseased midbrain" (~15 min)

    Ian Baker
    Dartmouth University
    "Magnetic Hyperthermia for Cancer Treatment" (~45 min)

    OCEM MMM July

    Monthly Microscopy Meeting: Live Cell Imaging and CLEM

    The OCEM has been invited by the Equipment Advisory Committee to submit an application for a High-resolution Analytical Field Emission Scanning Electron Microscope system with serial block face SEM (SBF-SEM) capability. SBF-FEG SEM will provide an advanced level 3D ultrastructural analysis that is entirely novel in New Zealand. In order to decide on the optimal configuration we have organised several presentations by providers and this month we are being visited by two speakers from Zeiss.

    The next meeting is at 9:00AM Thursday the 29th of June, in the seminar room of the Benham (Zoology) building.

    "Life happens in 3D and understanding biological context in the three dimensions has been a major driver in microscopy in the past decades. Whilst key biological functions are addressed by techniques like confocal fluorescence microscopy techniques, volume imaging by scanning electron microscopy, now offers an unprecedented understanding of the large ultrastructure biological objects. Correlating both imaging modalities in 3D is therefore a powerful way to link function to structure, even in complex biological systems."

    Bridge the Micro and Nano Worlds with Light and Electron Microscopy.
    Dr. Ruth Chalmers-Redman, Zeiss Singapore.

    Developments in Microscopy for Live Cell Imaging
    Dr. Rene Hessling, Zeiss Australia/New Zealand.

    There will also be a special presentation at 1.00PM in the Hunter Centre room 120 (upstairs).

    "For decades, confocal microscopy has offered us a glimpse into a 3D understanding of biological specimens. Recent years have brought us improved labelling techniques, faster acquisition, gentler imaging at higher resolution scales. These techniques rely on the cells of interest being labelled and therefore visible to the detection system. New advances in scanning electron microscopy offer volume imaging at an unprecedented scale allowing for a new understanding of the large ultrastructure of biological objects. With electron microscopy however, no cell can hide. Correlating both imaging modalities in 2D and 3D is therefore a powerful new technique to link function to structure, even in complex biological systems." Imaging 3D volumes in modern SEM and Correlation of Light and Electron Microscopy
    (Dr. Ruth Chalmers-Redman - ZEISS Singapore)

    New technologies in confocal microscopy for Live Cell Applications
    (Dr. Rene Hessling - ZEISS Australia/New Zealand)

    OCEM MMM June1 OCEM MMM June2

    Paper of the Month

    Fagerlund, R.D., Wilkinson, M.E., Klykov, O., Barendregt, A., Pearce, F.G., Kieper, S.N., Maxwell, H.W.R., Capolupo, A., Heck, A.J.R., Krause, K.L., Bostina, M., Scheltema, R.A., Staals, R.H.J. and Fineran, P.C. (2017) Spacer capture and integration by a type I-F Cas1-Cas2-3 CRISPR adaptation complex. Proceedings of the National Academy of Science of the United States of America. doi: 10.1073/pnas.1618421114.

    CRISPR

    First Round of EM Student Award Closes September 1st 2017

    Undergraduate and postgraduate students enrolled at the University of Otago are welcome to apply for the Electron Microscopy Student Research Award. The award provides 15 hours of electron microscopy time and aims to advance the initiation of new research projects that utilise electron microscopy. The deadline for applications is the 1st of September 2017. For more details visit the EM Student Award Webpage.



    Monthly Microscopy Meeting

    The next meeting is at 9:00AM Thursday the 25th of May, in the seminar room of the Benham (Zoology) building.
    There will also be a sales presentation at 1.OOPM on Thursday the 25th of May in room 120 of the Hunter Centre.

    Apreo: the most versatile high-performance SEM subnanometre resolution, and the best contrast from the most sensitive samples

    Dr. Ryan Shaw Materials & Structural Analysis (formerly FEI) Thermo Fisher Scientific

    OCEM MMM May

    Paper of the Month

    Yarragudi, S.B., Richter, R., Lee, H., Walker, G., Clarkson, A., Kumar, H. and Rizwan, S.B. (2017) Formulation of olfactory-targeted microparticles with tamarind seed polysaccharide to improve nose-to-brain transport of drugs. Carbohydrate Polymers, 163:216-226, doi.org/10.1016/j.carbpol.2017.01.044.

    Yarragudi_POM

    Monthly Microscopy Meeting

    The next meeting is at 9:00AM Thursday the 27th of April, in the seminar room of the Benham (Zoology) building.

    Round table: SEM in life and material sciences at Otago

    The OCEM intends to purchase an SEM to bring new capabilities to the unit. In order to decide on the optimal configuration, we have organised several presentations by providers. Last month we had JEOL and in the middle of May we will have Zeiss. At this month's microscopy meeting we will have a round table to analyse new technologies and their specific advantages for different projects (i.e. serial block facing SEM, array tomography, etc). Allan Mitchel will have a couple of slides presenting the options, after which we will try to discuss specific needs of each user.

    key words: SEM vs. TEM; large volumes vs. high resolution; large data sets and acquisition time; localization and colocalization; correlative microscopy.


    Paper of the Month

    Barad, Z., Grattan, D. and Leitch, B. (2017) NMDA Receptor Expression in the Thalamus of the Stargazer Model of Absence Epilepsy. Scientific Reports, 7:42926; doi: 10.1038/srep42926.

    Barad_POM

    Special Seminar

    1PM on Thursday 30th March in Room 123, first floor of the Huntre Centre (Great King Street).

    Anna Liew, JEOL Singapore
    "Frontiers in SEM, TEM Imaging and Microanalysis"

    Anna is a product specialist from JEOL Singapore. She will talk about about the rapidly developing technqiue of serial section scanning electron microscopy (a relatively quick technqiue for tracing specific structures through tissue over a long distance at high resolution, i.e neurons though brain tissue) and advances in elemental analysis detectors.


    Monthly Microscopy Meeting

    The next meeting is at 9:00AM Thursday the 30th of March, in the seminar room of the Benham (Zoology) building.

    Vitaly Lozbin, JEOL Australia
    "The latest application of FE SEM in Serial Block Face imaging and sub-10nm EDS microanalysis"

    Vitaly is a product specialist from JEOL Australia. He will talk about how an ultra high resolution field emission gun scanning electron microscope (FE SEM) equipped with Gatan 3View stage offers life science researchers the ability to obtain in-situ 3D data at remarkably fine depth resolution by operating a high-precision ultra microtome within the FE SEM specimen chamber. Optimisation of electron optics and utilisation of multi-EDS set up enables imaging and analysis of sub-10nm particles in Immuno-Electron Microscopy applications.

    OCEM MMM March

    Paper of the Month

    Hammer, N. (2017) Site-dependent acellularization effects explain altered tissue mechanics: ultrastructural insights. Folia Morphologica, DOI: 10.5603/FM.a2017.0007

    Hammer_POM


    Monthly Microscopy Meeting

    The next meeting is at 9:00AM Thursday the 23rd of February, in the seminar room of the Benham (Zoology) building.

    Laura Gumy, Department of Anatomy, University of Otago.
    "Visualising transport in neurons"

    Laura is a neurobiologist interested in the roles of cytoskeletal dynamics and protein trafficking in neurons - fundamental processes that when impaired can lead to the onset of neurodegenerative diseases or to deficits in nerve regeneration. She obtained her PhD from the Department of Biochemistry at the University of Cambridge, UK and subsequent post-doctoral training at the Centre for Brain Repair in Cambridge and at the University of Utrecht, the Netherlands with Casper Hoogenraad.

    OCEM MMM Sept