News, Events and Workshops

The list of news and events is by no means a comprehensive one, so if you have anything you'd like to add email Sharon at sharon.lequeux@anatomy.otago.ac.nz

  • News from 2016 and earlier


  • News from 2017:


    Monthly Microscopy Meeting

    The next meeting is at 9:00AM Thursday the 31st of August, in the seminar room of the Benham (Zoology) building. The OCEM intends to purchase an SEM to bring new capabilities to the unit. In order to decide on the optimal configuration, we have organised several presentations by providers. This month we will hear from Kamran Khajehpour from AXT, and Australian based supplier who, amongst at very wide range of EM preparation equipment, offer the Tescan range of scanning elecron microscopes and the Delmic range of CLEM platforms and cathodoluminescene detectors.

    There will also be a sales presentation at 1.OOPM on Thursday the 31st August in room 120 of the Hunter Centre.

    "Next Generation Microscopy Solutions for Life and Material Science Applications"


    Paper of the Month

    Miles, L.A., Burga, L.N., Gardner, E.E., Bostina, M., Poirier, J.T. and Rudin, C.M. (2017) Anthrax toxin receptor 1 is the cellular receptor for Seneca Valley virus. The Journal of Clinical Investigation, doi.org/10.1172/JCI93472.

    Seneca Valley Virus

    Cryo-EM Workshop - 24th July 2017

    Short Introduction to cryo-EM
    1030AM on Monday the 24th July and Tuesday the 1st of August 2017
    Room 207, Microbiology building. Enquires to mihnea.bostina@otago.ac.nz.



    Monthly Microscopy Meeting

    The next Microscopy Monthly Meeting is at 9:00AM Thursday the 27th of July, in the seminar room of the Benham (Zoology) building.

    Steve Seo
    Department of Anatomy
    "Understanding a synaptic connector in the healthy and diseased midbrain" (~15 min)

    Ian Baker
    Dartmouth University
    "Magnetic Hyperthermia for Cancer Treatment" (~45 min)

    OCEM MMM July

    Monthly Microscopy Meeting: Live Cell Imaging and CLEM

    The OCEM has been invited by the Equipment Advisory Committee to submit an application for a High-resolution Analytical Field Emission Scanning Electron Microscope system with serial block face SEM (SBF-SEM) capability. SBF-FEG SEM will provide an advanced level 3D ultrastructural analysis that is entirely novel in New Zealand. In order to decide on the optimal configuration we have organised several presentations by providers and this month we are being visited by two speakers from Zeiss.

    The next meeting is at 9:00AM Thursday the 29th of June, in the seminar room of the Benham (Zoology) building.

    "Life happens in 3D and understanding biological context in the three dimensions has been a major driver in microscopy in the past decades. Whilst key biological functions are addressed by techniques like confocal fluorescence microscopy techniques, volume imaging by scanning electron microscopy, now offers an unprecedented understanding of the large ultrastructure biological objects. Correlating both imaging modalities in 3D is therefore a powerful way to link function to structure, even in complex biological systems."

    Bridge the Micro and Nano Worlds with Light and Electron Microscopy.
    Dr. Ruth Chalmers-Redman, Zeiss Singapore.

    Developments in Microscopy for Live Cell Imaging
    Dr. Rene Hessling, Zeiss Australia/New Zealand.

    There will also be a special presentation at 1.00PM in the Hunter Centre room 120 (upstairs).

    "For decades, confocal microscopy has offered us a glimpse into a 3D understanding of biological specimens. Recent years have brought us improved labelling techniques, faster acquisition, gentler imaging at higher resolution scales. These techniques rely on the cells of interest being labelled and therefore visible to the detection system. New advances in scanning electron microscopy offer volume imaging at an unprecedented scale allowing for a new understanding of the large ultrastructure of biological objects. With electron microscopy however, no cell can hide. Correlating both imaging modalities in 2D and 3D is therefore a powerful new technique to link function to structure, even in complex biological systems." Imaging 3D volumes in modern SEM and Correlation of Light and Electron Microscopy
    (Dr. Ruth Chalmers-Redman - ZEISS Singapore)

    New technologies in confocal microscopy for Live Cell Applications
    (Dr. Rene Hessling - ZEISS Australia/New Zealand)

    OCEM MMM June1 OCEM MMM June2

    Paper of the Month

    Fagerlund, R.D., Wilkinson, M.E., Klykov, O., Barendregt, A., Pearce, F.G., Kieper, S.N., Maxwell, H.W.R., Capolupo, A., Heck, A.J.R., Krause, K.L., Bostina, M., Scheltema, R.A., Staals, R.H.J. and Fineran, P.C. (2017) Spacer capture and integration by a type I-F Cas1-Cas2-3 CRISPR adaptation complex. Proceedings of the National Academy of Science of the United States of America. doi: 10.1073/pnas.1618421114.

    CRISPR

    First Round of EM Student Award Closes September 1st 2017

    Undergraduate and postgraduate students enrolled at the University of Otago are welcome to apply for the Electron Microscopy Student Research Award. The award provides 15 hours of electron microscopy time and aims to advance the initiation of new research projects that utilise electron microscopy. The deadline for applications is the 1st of September 2017. For more details visit the EM Student Award Webpage.



    Monthly Microscopy Meeting

    The next meeting is at 9:00AM Thursday the 25th of May, in the seminar room of the Benham (Zoology) building.
    There will also be a sales presentation at 1.OOPM on Thursday the 25th of May in room 120 of the Hunter Centre.

    Apreo: the most versatile high-performance SEM subnanometre resolution, and the best contrast from the most sensitive samples

    Dr. Ryan Shaw Materials & Structural Analysis (formerly FEI) Thermo Fisher Scientific

    OCEM MMM May

    Paper of the Month

    Yarragudi, S.B., Richter, R., Lee, H., Walker, G., Clarkson, A., Kumar, H. and Rizwan, S.B. (2017) Formulation of olfactory-targeted microparticles with tamarind seed polysaccharide to improve nose-to-brain transport of drugs. Carbohydrate Polymers, 163:216-226, doi.org/10.1016/j.carbpol.2017.01.044.

    Yarragudi_POM

    Monthly Microscopy Meeting

    The next meeting is at 9:00AM Thursday the 27th of April, in the seminar room of the Benham (Zoology) building.

    Round table: SEM in life and material sciences at Otago

    The OCEM intends to purchase an SEM to bring new capabilities to the unit. In order to decide on the optimal configuration, we have organised several presentations by providers. Last month we had JEOL and in the middle of May we will have Zeiss. At this month's microscopy meeting we will have a round table to analyse new technologies and their specific advantages for different projects (i.e. serial block facing SEM, array tomography, etc). Allan Mitchel will have a couple of slides presenting the options, after which we will try to discuss specific needs of each user.

    key words: SEM vs. TEM; large volumes vs. high resolution; large data sets and acquisition time; localization and colocalization; correlative microscopy.


    Paper of the Month

    Barad, Z., Grattan, D. and Leitch, B. (2017) NMDA Receptor Expression in the Thalamus of the Stargazer Model of Absence Epilepsy. Scientific Reports, 7:42926; doi: 10.1038/srep42926.

    Barad_POM

    Special Seminar

    1PM on Thursday 30th March in Room 123, first floor of the Huntre Centre (Great King Street).

    Anna Liew, JEOL Singapore
    "Frontiers in SEM, TEM Imaging and Microanalysis"

    Anna is a product specialist from JEOL Singapore. She will talk about about the rapidly developing technqiue of serial section scanning electron microscopy (a relatively quick technqiue for tracing specific structures through tissue over a long distance at high resolution, i.e neurons though brain tissue) and advances in elemental analysis detectors.


    Monthly Microscopy Meeting

    The next meeting is at 9:00AM Thursday the 30th of March, in the seminar room of the Benham (Zoology) building.

    Vitaly Lozbin, JEOL Australia
    "The latest application of FE SEM in Serial Block Face imaging and sub-10nm EDS microanalysis"

    Vitaly is a product specialist from JEOL Australia. He will talk about how an ultra high resolution field emission gun scanning electron microscope (FE SEM) equipped with Gatan 3View stage offers life science researchers the ability to obtain in-situ 3D data at remarkably fine depth resolution by operating a high-precision ultra microtome within the FE SEM specimen chamber. Optimisation of electron optics and utilisation of multi-EDS set up enables imaging and analysis of sub-10nm particles in Immuno-Electron Microscopy applications.

    OCEM MMM March

    Paper of the Month

    Hammer, N. (2017) Site-dependent acellularization effects explain altered tissue mechanics: ultrastructural insights. Folia Morphologica, DOI: 10.5603/FM.a2017.0007

    Hammer_POM


    Monthly Microscopy Meeting

    The next meeting is at 9:00AM Thursday the 23rd of February, in the seminar room of the Benham (Zoology) building.

    Laura Gumy, Department of Anatomy, University of Otago.
    "Visualising transport in neurons"

    Laura is a neurobiologist interested in the roles of cytoskeletal dynamics and protein trafficking in neurons - fundamental processes that when impaired can lead to the onset of neurodegenerative diseases or to deficits in nerve regeneration. She obtained her PhD from the Department of Biochemistry at the University of Cambridge, UK and subsequent post-doctoral training at the Centre for Brain Repair in Cambridge and at the University of Utrecht, the Netherlands with Casper Hoogenraad.

    OCEM MMM Sept